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Mouse GUSb (Glucuronidase Beta) ELISA Kit

CAT: 0855-ELK2773-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK2773-01Size:48 Tests
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse GUSb. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse GUSb. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse GUSb, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse GUSb in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
GUS-B; MPS7; Beta-G1
UniProt
P12265
Reactivity
Mouse
Field of Research
Signal transduction; Enzyme & Kinase; Metabolic pathway
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse GUSb. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse GUSb. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse GUSb, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse GUSb in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
4000 pg/mL
Sample Type
Serum, plasma, tissue homogenates and other biological fluids
Detection Range
62.5-4000 pg/mL
Sensitivity
27.6 pg/mL

UniProtKB · P12265

Beta-glucuronidase

BGLR_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
P12265
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Gusb
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
—
EC number
3.2.1.31
Processing
Precursor
Secondary accessions
Q61601, Q64473, Q64474, Q6IR10
Protein keywords

Cellular component

Endoplasmic reticulumLysosome

PTM

Glycoprotein

Molecular function

GlycosidaseHydrolase

Technical term

Reference proteome

Domain

Signal

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