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Rat GUSb (Glucuronidase Beta) ELISA Kit

CAT: 0855-ELK5792-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK5792-01Size:48 Tests
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat GUSb. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat GUSb. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat GUSb, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat GUSb in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
GUS-B; MPS7; Beta-G1
UniProt
P06760
Reactivity
Rat
Field of Research
Signal transduction; Enzyme & Kinase; Metabolic pathway
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat GUSb. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat GUSb. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat GUSb, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat GUSb in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
20 ng/mL
Sample Type
Serum, plasma, tissue homogenates and other biological fluids
Detection Range
0.32-20 ng/mL
Sensitivity
0.116 ng/mL

UniProtKB · P06760

Beta-glucuronidase

BGLR_RAT · Rattus norvegicus

View on UniProt ↗
Primary accession
P06760
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Gusb
Protein existence
1: Evidence at protein level
Organism
Rattus norvegicus (Rat)
Taxonomy ID
10116
Alternative names
—
EC number
3.2.1.31
Processing
Precursor
Secondary accessions
—
Protein keywords

PTM

Glycoprotein

Molecular function

GlycosidaseHydrolase

Cellular component

Lysosome

Technical term

Reference proteome

Domain

Signal

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