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Human ELA3B (Elastase 3B) ELISA Kit

CAT: 0855-ELK3615-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK3615-01Size:48 Tests
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ELA3B. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ELA3B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ELA3B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ELA3B in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
CELA3B; Chymotrypsin Like Elastase Family Member 3B; Protease E; Fecal Elastase; Elastase 1; Cholesterol-Binding Pancreatic Protease; Pancreatic Endopeptidase E
UniProt
P08861
Reactivity
Human
Field of Research
Enzyme & Kinase
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ELA3B. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ELA3B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ELA3B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ELA3B in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
20 ng/mL
Sample Type
Serum, plasma and other biological fluids.
Detection Range
0.32-20 ng/mL
Sensitivity
0.129 ng/mL

UniProtKB · P08861

Chymotrypsin-like elastase family member 3B

CEL3B_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P08861
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
CELA3B
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
3.4.21.70
Processing
Precursor
Secondary accessions
B2RE44, P11423, Q5VU28, Q5VU29, Q5VU30
Protein keywords

Technical term

Direct protein sequencingProteomics identificationReference proteome

PTM

Disulfide bondGlycoproteinZymogen

Molecular function

HydrolaseProteaseSerine protease

Domain

Signal

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