Human ELA2B (Elastase 2B) Microsample ELISA Kit

CAT: 0855-ELK3804MS-01Size: 48 TestsDry Ice: NoHazardous: No
CAT#:0855-ELK3804MS-01Size:48 Tests
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Product Name Alternative
CELA2B; Chymotrypsin-Like Elastase Family Member 2B; Pancreatic Elastase IIB
UniProt
P08218
Reactivity
Human
Field of Research
Enzyme & Kinase
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ELA2B. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ELA2B. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ELA2B, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ELA2B in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
20 ng/mL
Sample Type
Serum, plasma, tissue homogenates and other biological fluids.
Detection Range
0.32-20 ng/mL
Sensitivity
0.11 ng/mL

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