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Human KLb (Klotho Beta) ELISA Kit

CAT: 0855-ELK3813-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK3813-01Size:48 Tests
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human KLb. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human KLb. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human KLb, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human KLb in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
Klotho beta-like protein
UniProt
Q86Z14
Reactivity
Human
Field of Research
Metabolic pathway
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human KLb. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human KLb. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human KLb, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human KLb in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
5000 pg/mL
Sample Type
Serum, plasma and other biological fluids
Detection Range
78.13-5000 pg/mL
Sensitivity
29 pg/mL

UniProtKB · Q86Z14

Beta-klotho

KLOTB_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
Q86Z14
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
KLB
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
BKL, BetaKlotho
EC number
—
Processing
—
Secondary accessions
Q2M3K8
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

Cellular component

Cell membraneMembrane

PTM

Glycoprotein

Domain

RepeatTransmembraneTransmembrane helix

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