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Rat KL (Klotho) ELISA Kit

CAT: 0855-ELK6278-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK6278-01Size:48 Tests
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat KL. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat KL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat KL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat KL in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
Kl ; KLOT; Klotho peptide
UniProt
Q9Z2Y9
Reactivity
Rat
Field of Research
Metabolic pathway
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat KL. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat KL. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat KL, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat KL in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
20 ng/mL
Sample Type
Serum, plasma, tissue homogenates and other biological fluids
Detection Range
0.32-20 ng/mL
Sensitivity
0.113 ng/mL

UniProtKB · Q9Z2Y9

Klotho

KLOT_RAT · Rattus norvegicus

View on UniProt ↗
Primary accession
Q9Z2Y9
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Kl
Protein existence
1: Evidence at protein level
Organism
Rattus norvegicus (Rat)
Taxonomy ID
10116
Alternative names
—
EC number
3.2.1.31
Processing
Precursor
Secondary accessions
—
Protein keywords

Cellular component

Cell membraneMembraneSecreted

PTM

Glycoprotein

Molecular function

GlycosidaseHydrolase

Technical term

Reference proteome

Domain

RepeatSignalTransmembraneTransmembrane helix

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