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Human CCNB (Cyclin B) ELISA Kit

CAT: 0855-ELK4082-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK4082-01Size:48 Tests
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CCNB. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CCNB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CCNB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CCNB in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
CCNB1; CCN-B; G2/Mitotic-Specific Cyclin B1
UniProt
P14635
Reactivity
Human
Field of Research
Tumor immunity
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CCNB. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CCNB. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CCNB, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CCNB in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
20 ng/mL
Sample Type
Tissue homogenates, cell lysates and other biological fluids
Detection Range
0.32-20 ng/mL
Sensitivity
0.114 ng/mL

UniProtKB · P14635

G2/mitotic-specific cyclin-B1

CCNB1_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P14635
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
CCNB1
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
—
Secondary accessions
A8K066, Q5TZP9
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

PTM

AcetylationPhosphoproteinUbl conjugation

Coding sequence diversity

Alternative splicing

Biological process

Cell cycleCell divisionMitosis

Molecular function

Cyclin

Cellular component

CytoplasmCytoskeletonNucleus

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