Products for Research Use Only
RegisterLogin

Human CCNE1 (Cyclin E1) ELISA Kit

CAT: 0855-ELK0851-01Size: 48 TestsDry Ice: NoHazardous: No
Product image 1
1 / 1
CAT#:0855-ELK0851-01Size:48 Tests
Selected
24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Quick Request Actions
Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CCNE1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CCNE1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CCNE1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CCNE1 in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
3938; BG:DS07108.3; br37; cdi7; Cdi7; CDI7; CG3938; Cyc; Cyc E; Cyc-E; cycE; CYCE; CycEI; CyclE; cyclin E; cyclin-E; cycline; cyclinE; CyE-1; CyeE; D-CycE; dCycE; dm-cycE; DmcycE; DmCycE; DmcyclinE; Dmel\CG3938; fond; l (2) 05206; l (2) 35Dd; l (2) br37; l (2) k02514; l (2) k02602; l (2) k05007; l35Dd
UniProt
P24864
Reactivity
Human
Field of Research
Metabolic pathway; Developmental science
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CCNE1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CCNE1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CCNE1, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CCNE1 in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
5000 pg/mL
Sample Type
Tissue homogenates, cell lysates, cell culture supernates and other biological fluids
Detection Range
78.13-5000 pg/mL
Sensitivity
29.7 pg/mL

UniProtKB · P24864

G1/S-specific cyclin-E1

CCNE1_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P24864
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
CCNE1
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
—
Secondary accessions
A8K684, Q14091, Q8NFG1, Q92501, Q9UD21
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

Coding sequence diversity

Alternative splicing

Biological process

Cell cycleCell division

Molecular function

Cyclin

Cellular component

Nucleus

PTM

PhosphoproteinUbl conjugation

Related Products

CatalogName
ES2082-01Cyclin E1 rabbit pAb
ELK4081-01Human CCNA1 (Cyclin A1) ELISA Kit
ELK1487-01Human CCND1 (Cyclin D1) ELISA Kit
ES7920-01Cyclin E1 Rabbit Polyclonal Antibody
ES2083-01Cyclin E1 Rabbit Polyclonal Antibody
ELK7837-01E1 (Estrone) ELISA Kit
ES7919-01Cyclin E1 (phospho Thr77) rabbit pAb
ES1295-01Cyclin E1 (phospho Thr395) rabbit pAb
ELK11277-01Human RvE1 (Resolvin E1) ELISA Kit
ELK7740-01Rat CCND1 (Cyclin D1) ELISA Kit

Alternative Products

CatalogName
abx259513Human Cyclin E1 (CCNE1) ELISA Kit
MBS8820225-01Human CCNE1 (Cyclin E1) ELISA Kit
GTR18856380-01Mouse Cyclin-E1 (CCNE1) ELISA Kit
FY-ER5540Rat Ccne1 (Cyclin-E1) ELISA Kit
abx354042-01Rat Cyclin E1 (CCNE1) ELISA Kit
E-EL-M1307ELISA kit for Mouse CCNE1 (Cyclin-E1)
FY-EM5539Mouse Ccne1 (Cyclin-E1) ELISA Kit
GTR18856378-01Rat Cyclin-E1 (CCNE1) ELISA Kit
E-EL-R1126ELISA kit for Rat CCNE1 (Cyclin-E1)
abx353182-01Mouse Cyclin E1 (CCNE1) ELISA Kit

Popular Products