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Human CTRB2 (Chymotrypsinogen B2) ELISA Kit

CAT: 0855-ELK4103-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK4103-01Size:48 Tests
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CTRB2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CTRB2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CTRB2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CTRB2 in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
Chymotrypsin B2 chain C; CTRB2
UniProt
Q6GPI1
Reactivity
Human
Field of Research
Enzyme & Kinase
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human CTRB2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human CTRB2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human CTRB2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human CTRB2 in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
100 ng/mL
Sample Type
Serum, plasma and other biological fluids.
Detection Range
1.57-100 ng/mL
Sensitivity
0.63 ng/mL

UniProtKB · Q6GPI1

Chymotrypsinogen B2

CTRB2_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
Q6GPI1
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
CTRB2
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
3.4.21.1
Processing
Precursor
Secondary accessions
A8K707
Protein keywords

Biological process

Digestion

PTM

Disulfide bondZymogen

Molecular function

HydrolaseProteaseSerine protease

Technical term

Proteomics identificationReference proteome

Cellular component

Secreted

Domain

Signal

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