VB2 (Vitamin B2) ELISA Kit

• Description:

  This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with VB2 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to VB2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of VB2 in the samples is then determined by comparing the OD of the samples to the standard curve.

• Product Name Alternative:

  Riboflavin; Riboflavine

• Reactivity:

  General

• Field of Research:

  Nutrition metabolism; Hormone metabolism

• Assay Type:

  Competitive Inhibition

• Assay Principle:

  This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with VB2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to VB2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of VB2 in the samples is then determined by comparing the OD of the samples to the standard curve.

• Assay Performance Time:

  2h

• Standard:

  600 ng/mL

• Sample Type:

  Serum, plasma, cell lysates, cell culture supernates and other biological fluids

• Detection Range:

  9.38-600 ng/mL

• Sensitivity:

  2.45 ng/mL