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Human EXOSC2 (Exosome Component 2) ELISA Kit

CAT: 0855-ELK4639-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK4639-01Size:48 Tests
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human EXOSC2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human EXOSC2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human EXOSC2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human EXOSC2 in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
RRP4; Rrp4p; hRrp4p; p7; Ribosomal RNA-processing protein 4
UniProt
Q13868
Reactivity
Human
Field of Research
Enzyme & Kinase
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human EXOSC2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human EXOSC2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human EXOSC2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human EXOSC2 in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
10 ng/mL
Sample Type
Tissue homogenates, cell lysates and other biological fluids
Detection Range
0.16-10 ng/mL
Sensitivity
0.059 ng/mL

UniProtKB · Q13868

Exosome complex component RRP4

EXOS2_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
Q13868
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
EXOSC2
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
—
Secondary accessions
A3KFL3, A3KFL4, B4DKK6, Q9NUY4
Protein keywords

Technical term

3D-structureProteomics identificationReference proteome

Coding sequence diversity

Alternative splicing

Cellular component

CytoplasmExosomeNucleus

Disease

DeafnessDisease variantDwarfismRetinitis pigmentosa

PTM

Phosphoprotein

Molecular function

RNA-binding

Biological process

rRNA processing

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