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Human C2 (Complement Component 2) ELISA Kit

CAT: 0855-ELK11105-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK11105-01Size:48 Tests
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human C2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human C2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human C2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human C2 in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
CO2; C3/C5 convertase
UniProt
P06681
Reactivity
Human
Field of Research
Signal transduction; Infection immunity; Immune molecule
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human C2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human C2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human C2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human C2 in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
500 ng/mL
Sample Type
Serum, plasma and other biological fluids
Detection Range
7.82-500 ng/mL
Sensitivity
3.2 ng/mL

UniProtKB · P06681

Complement C2

CO2_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P06681
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
C2
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
B4DPF3, B4DV20, E9PFN7, O19694, Q13904
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Disease

Age-related macular degenerationDisease variant

Coding sequence diversity

Alternative splicing

Biological process

Complement pathwayImmunityInnate immunity

PTM

Disulfide bondGlycoprotein

Molecular function

HydrolaseProteaseSerine protease

Ligand

Metal-binding

Domain

RepeatSignalSushi

Cellular component

Secreted

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