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Mouse HTT (Huntingtin) ELISA Kit

CAT: 0855-ELK6311-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK6311-01Size:48 Tests
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse HTT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse HTT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse HTT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse HTT in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
IT15; HD; Interesting Transcript 15; Huntington disease protein
UniProt
P42859
Reactivity
Mouse
Field of Research
Signal transduction
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse HTT. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse HTT. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse HTT, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse HTT in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
10 ng/mL
Sample Type
Tissue homogenates, cell lysates and other biological fluids
Detection Range
0.16-10 ng/mL
Sensitivity
0.061 ng/mL

UniProtKB · P42859

Huntingtin

HD_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
P42859
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Htt
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
—
EC number
—
Processing
—
Secondary accessions
—
Protein keywords

PTM

AcetylationLipoproteinMyristatePhosphoprotein

Coding sequence diversity

Alternative splicing

Cellular component

CytoplasmCytoplasmic vesicleNucleus

Technical term

Reference proteome

Domain

Repeat

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