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Mouse BGN (Biglycan) ELISA Kit

CAT: 0855-ELK6496-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK6496-01Size:48 Tests
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse BGN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse BGN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse BGN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse BGN in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
DSPG1; PG-S1; PGI; SLRR1A; Biglycan Proteoglycan; Bone/cartilage proteoglycan I
UniProt
P28653
Reactivity
Mouse
Field of Research
Reproductive science
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Mouse BGN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse BGN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Mouse BGN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse BGN in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
200 ng/mL
Sample Type
Serum, plasma and other biological fluids
Detection Range
3.13-200 ng/mL
Sensitivity
1.41 ng/mL

UniProtKB · P28653

Biglycan

PGS1_MOUSE · Mus musculus

View on UniProt ↗
Primary accession
P28653
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Bgn
Protein existence
1: Evidence at protein level
Organism
Mus musculus (Mouse)
Taxonomy ID
10090
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
Q61355
Protein keywords

PTM

Disulfide bondGlycoproteinProteoglycan

Cellular component

Extracellular matrixSecreted

Domain

Leucine-rich repeatRepeatSignal

Technical term

Reference proteome

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