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Human BGN (Biglycan) ELISA Kit

CAT: 0855-ELK3436-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK3436-01Size:48 Tests
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human BGN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human BGN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human BGN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human BGN in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
DSPG1; PG-S1; PGI; SLRR1A; Biglycan Proteoglycan; Bone/cartilage proteoglycan I
UniProt
P21810
Reactivity
Human
Field of Research
Reproductive science
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human BGN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human BGN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human BGN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human BGN in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
20 ng/mL
Sample Type
Serum, plasma and other biological fluids
Detection Range
0.32-20 ng/mL
Sensitivity
0.131 ng/mL

UniProtKB · P21810

Biglycan

PGS1_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
P21810
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
BGN
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
D3DWU3, P13247
Protein keywords

Disease

Aortic aneurysmDisease variantDwarfism

Technical term

Direct protein sequencingProteomics identificationReference proteome

PTM

Disulfide bondGlycoproteinProteoglycan

Cellular component

Extracellular matrixSecreted

Domain

Leucine-rich repeatRepeatSignal

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