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Human EPG (Epigen) ELISA Kit

CAT: 0855-ELK6583-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK6583-01Size:48 Tests
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human EPG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human EPG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human EPG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human EPG in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
EPGN; Epithelial Mitogen Homolog
UniProt
Q6UW88
Reactivity
Human
Field of Research
Cytokine
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human EPG. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human EPG. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human EPG, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human EPG in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
20 ng/mL
Sample Type
Tissue homogenates, cell lysates and other biological fluids
Detection Range
0.32-20 ng/mL
Sensitivity
0.111 ng/mL

UniProtKB · Q6UW88

Epigen

EPGN_HUMAN · Homo sapiens

View on UniProt ↗
Primary accession
Q6UW88
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
EPGN
Protein existence
1: Evidence at protein level
Organism
Homo sapiens (Human)
Taxonomy ID
9606
Alternative names
—
EC number
—
Processing
Precursor
Secondary accessions
A1BMM3, A1BMM4, A1BMM5, A1BMM6, A1BMM7, A1BMM8, A8K090
Protein keywords

Technical term

3D-structureDirect protein sequencingProteomics identificationReference proteome

Coding sequence diversity

Alternative splicing

PTM

Disulfide bondGlycoprotein

Domain

EGF-like domainSignalTransmembraneTransmembrane helix

Molecular function

Growth factor

Cellular component

MembraneSecreted

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