Rat APLN (Apelin) ELISA Kit

• Description:

  This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat APLN protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat APLN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat APLN in the samples is then determined by comparing the OD of the samples to the standard curve.

• Product Name Alternative:

  XNPEP2; APEL; APJ endogenous ligand

• Gene Aliases:

  XNPEP2; APEL; APJ endogenous ligand

• UniProt:

  Q9R0R3

• Reactivity:

  Rat

• Field of Research:

  Endocrinology

• Assay Type:

  Competitive Inhibition

• Assay Principle:

  This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat APLN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat APLN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat APLN in the samples is then determined by comparing the OD of the samples to the standard curve.

• Assay Performance Time:

  2 H

• Standard:

  1000 pg/mL

• Sample Type:

  Serum, plasma, cell lysates, cell culture supernates and other biological fluids

• Detection Range:

  15.63 - 1000 pg/mL

• Sensitivity:

  4.36 pg/mL

• Species:

  Rat

• CAS Number:

  7732-18-5