Mouse APLN (Apelin) ELISA Kit

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Description

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse APLN protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse APLN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse APLN in the samples is then determined by comparing the OD of the samples to the standard curve.

Product Name Alternative

XNPEP2; APEL; APJ endogenous ligand

UniProt

Q9R0R4

Reactivity

Mouse

Field of Research

Endocrinology

Assay Type

Competitive Inhibition

Assay Principle

This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Mouse APLN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Mouse APLN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Mouse APLN in the samples is then determined by comparing the OD of the samples to the standard curve.

Assay Performance Time

Standard

8000 pg/mL

Sample Type

Serum, plasma and other biological fluids

Detection Range

125-8000 pg/mL

Sensitivity

34.8 pg/mL

UniProtKB · Q9R0R4

Apelin

APEL_MOUSE · Mus musculus

View on UniProt ↗

Primary accession: Q9R0R4
Review status: UniProtKB reviewed (Swiss-Prot)
Gene: Apln
Protein existence: 1: Evidence at protein level
Organism: Mus musculus (Mouse)
Taxonomy ID: 10090
Alternative names: —
EC number: —
Processing: Precursor
Secondary accessions: —

Protein keywords

Biological process

AngiogenesisGastrulation

PTM

Cleavage on pair of basic residues

Molecular function

Developmental proteinHormone

Technical term

Reference proteome

Cellular component

Secreted

Domain

Signal

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