Horse APLN (Apelin) ELISA Kit

• Description:

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Horse APLN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Horse APLN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Horse APLN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Horse APLN in the samples is then determined by comparing the OD of the samples to the standard curve.

• Product Name Alternative:

  XNPEP2; APEL; APJ enenous ligand

• Gene Aliases:

  XNPEP2; APEL; APJ en犬enous ligand

• Reactivity:

  Horse

• Field of Research:

  Endocrinology

• Assay Type:

  Sandwich

• Assay Principle:

  The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Horse APLN. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Horse APLN. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Horse APLN, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Horse APLN in the samples is then determined by comparing the OD of the samples to the standard curve.

• Assay Performance Time:

  3.5 H

• Standard:

  8000 pg/mL

• Sample Type:

  Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

• Detection Range:

  125 - 8000 pg/mL

• Sensitivity:

  46 pg/mL

• Species:

  Horse

• CAS Number:

  7732-18-5