E1 (Estrone) ELISA Kit

• Description:

  This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with E1 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to E1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of E1 in the samples is then determined by comparing the OD of the samples to the standard curve.

• Product Name Alternative:

  Oestrone

• Reactivity:

  General

• Field of Research:

  Metabolic pathway; Endocrinology; Reproductive science; Genetic science; Hormone metabolism

• Assay Type:

  Competitive Inhibition

• Assay Principle:

  This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with E1. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to E1. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of E1 in the samples is then determined by comparing the OD of the samples to the standard curve.

• Assay Performance Time:

  2h

• Standard:

  2000 pg/mL

• Sample Type:

  Serum, plasma and other biological fluids

• Detection Range:

  31.25-2000 pg/mL

• Sensitivity:

  9.19 pg/mL