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Estrogen ELISA Kit

CAT: 0855-ELK9031-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK9031-01Size:48 Tests
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Description
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Estrogen protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Estrogen. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Estrogen in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
E ; Estrogen
Reactivity
General
Field of Research
Reproductive science
Assay Type
Competitive Inhibition
Assay Principle
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Estrogen. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Estrogen. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Estrogen in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
2h
Standard
1000 pg/mL
Sample Type
Serum, plasma, other biological fluids
Detection Range
15.625-1000 pg/mL
Sensitivity
4.01 pg/mL

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