VB7 (Vitamin B7) ELISA Kit

VB7 (Vitamin B7) ELISA Kit

• Description: This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with VB7 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to VB7. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of VB7 in the samples is then determined by comparing the OD of the samples to the standard curve.
• CAS Number: 7732-18-5
• Gene Aliases: Biotin; Vitamin H; Coenzyme R; Biopeiderm
• Field of Research: Nutrition metabolism; Hormone metabolism.
• Assay Principle: Competitive Inhibition ELISA
• Assay Performance Time: 2.5h
• Standard: 20000 pg/mL
• Sample Type: Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids
• Detection Range: 312.5-20000 pg/mL
• Sensitivity: 91.9 pg/mL