VD3 (Vitamin D3) ELISA Kit

• Description:

  This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with VD3 protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to VD3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of VD3 in the samples is then determined by comparing the OD of the samples to the standard curve.

• Product Name Alternative:

  Cholecalciferol; Toxiferol; Activated 7-Dehydrocholesterol

• Reactivity:

  General

• Field of Research:

  Metabolic pathway; Reproductive science; Nutrition metabolism; Bone metabolism

• Assay Type:

  Competitive Inhibition

• Assay Principle:

  This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with VD3. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to VD3. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of VD3 in the samples is then determined by comparing the OD of the samples to the standard curve.

• Assay Performance Time:

  2h

• Standard:

  400 ng/mL

• Sample Type:

  Serum, plasma and other biological fluids

• Detection Range:

  6.25-400 ng/mL

• Sensitivity:

  2.12 ng/mL