Rat Hyp (Hydroxyproline) ELISA Kit

• Description:

  This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat Hyp protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat Hyp. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat Hyp in the samples is then determined by comparing the OD of the samples to the standard curve.

• Product Name Alternative:

  (2S,4R) -4-Hydroxyproline; L-Hydroxyproline (C5H9O3N)

• Gene Aliases:

  (2S,4R)-4-Hydroxyproline; L-Hydroxyproline(C5H9O3N)

• Reactivity:

  Rat

• Field of Research:

  Metabolic pathway, Nutrition metabolism

• Assay Type:

  Competitive Inhibition

• Assay Principle:

  This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Rat Hyp. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat Hyp. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat Hyp in the samples is then determined by comparing the OD of the samples to the standard curve.

• Assay Performance Time:

  2 H

• Standard:

  5000 ng/mL

• Sample Type:

  Serum, plasma, cell lysates, cell culture supernates and other biological fluids

• Detection Range:

  78.13 - 5000 ng/mL

• Sensitivity:

  27.3 ng/mL

• Species:

  Rat

• CAS Number:

  7732-18-5