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Rat STOML2 ELISA Kit

CAT: 0855-ELK8403-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK8403-01Size:48 Tests
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Description
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat STOML2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat STOML2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat STOML2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat STOML2 in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
SLP-2; Paratarg-7; EPB72-like protein 2; Paraprotein target 7
UniProt
Q4FZT0
Reactivity
Rat
Field of Research
Tumor marker
Assay Type
Sandwich
Assay Principle
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Rat STOML2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Rat STOML2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Rat STOML2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Rat STOML2 in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
3.5h
Standard
10 ng/mL
Sample Type
Tissue homogenates, cell lysates and other biological fluids
Detection Range
0.156-10 ng/mL
Sensitivity
0.053 ng/mL

UniProtKB · Q4FZT0

Stomatin-like protein 2, mitochondrial

STML2_RAT · Rattus norvegicus

View on UniProt ↗
Primary accession
Q4FZT0
Review status
UniProtKB reviewed (Swiss-Prot)
Gene
Stoml2
Protein existence
1: Evidence at protein level
Organism
Rattus norvegicus (Rat)
Taxonomy ID
10116
Alternative names
SLP-2
EC number
—
Processing
Precursor
Secondary accessions
—
Protein keywords

PTM

AcetylationLipoproteinPhosphoprotein

Cellular component

Cell membraneCytoplasmCytoskeletonMembraneMitochondrionMitochondrion inner membrane

Domain

Coiled coilTransit peptide

Technical term

Direct protein sequencingReference proteome

Ligand

Lipid-binding

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