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Hyp (Hydroxyproline) ELISA Kit

CAT: 0855-ELK7835-01Size: 48 TestsDry Ice: NoHazardous: No
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CAT#:0855-ELK7835-01Size:48 Tests
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Hyp protein. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Hyp. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Hyp in the samples is then determined by comparing the OD of the samples to the standard curve.
Product Name Alternative
(2S,4R) -4-Hydroxyproline; L-Hydroxyproline (C5H9O3N)
Reactivity
General
Field of Research
Metabolic pathway; Nutrition metabolism
Assay Type
Competitive Inhibition
Assay Principle
This assay employs the competitive inhibition enzyme immunoassay technique. The microtiter plate provided in this kit has been pre-coated with Hyp. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Hyp. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Hyp in the samples is then determined by comparing the OD of the samples to the standard curve.
Assay Performance Time
2h
Standard
5000 ng/mL
Sample Type
Serum, plasma, cell lysates, cell culture supernates and other biological fluids
Detection Range
78.13-5000 ng/mL
Sensitivity
25.4 ng/mL

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