Human ITGb2 (Integrin Beta 2) Microsample ELISA Kit
CAT:
855-ELK4937MS-01
Size:
48 Tests
Price:
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- Availability: 24/48H Stock Items & 2 to 6 Weeks non Stock Items.
- Dry Ice Shipment: No


Human ITGb2 (Integrin Beta 2) Microsample ELISA Kit
Product Name Alternative:
CD18; ITG-B2; LAD; LFA1; MAC1; LCAMB; MF17; LFAb; Complement Component 3 Receptor 3 And 4 Subunit; Lymphocyte Function-Associated Antigen 1; Macrophage Antigen 1UniProt:
P05107Reactivity:
HumanField of Research:
Signal transduction, CD & Adhesion molecule, Tumor immunityAssay Type:
SandwichAssay Principle:
The test principle applied in this kit is Sandwich enzyme immunoassay. The microtiter plate provided in this kit has been pre-coated with an antibody specific to Human ITGb2. Standards or samples are added to the appropriate microtiter plate wells then with a biotin-conjugated antibody specific to Human ITGb2. Next, Avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. After TMB substrate solution is added, only those wells that contain Human ITGb2, biotin-conjugated antibody and enzyme-conjugated Avidin will exhibit a change in color. The enzyme-substrate reaction is terminated by the addition of sulphuric acid solution and the color change is measured spectrophotometrically at a wavelength of 450nm ± 10nm. The concentration of Human ITGb2 in the samples is then determined by comparing the OD of the samples to the standard curve.Assay Performance Time:
3.5 HStandard:
20 ng/mLSample Type:
Tissue homogenates, cell lysates, cell culture supernates and other biological fluids.Detection Range:
0.32 - 20 ng/mLSensitivity:
0.113 ng/mL