E2F-2 (Acetyl Lys122) rabbit pAb

• Background:

  Function:Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from g1 to s phase. E2F-2 binds specifically to RB1 protein, in a cell-cycle dependent manner., PTM:Phosphorylated by CDK2 and cyclin A-CDK2 in the S-phase., similarity:Belongs to the E2F/DP family., subunit:Component of the DRTF1/E2F transcription factor complex. Forms heterodimers with DP family members. The E2F-2 complex binds specifically hypophosphorylated retinoblastoma protein RB1. During the cell cycle, RB1 becomes phosphorylated in mid-to-late G1 phase, detaches from the DRTF1/E2F complex, rendering E2F transcriptionally active. Viral oncoproteins, notably E1A, T-antigen and HPV E7, are capable of sequestering RB protein, thus releasing the active complex. Binds EAPP., tissue specificity:Highest level of expression is found in placenta, low levels are found in lung. Found as well in many immortalized cell lines derived from tumor samples.

• Description:

  Function: Transcription activator that binds DNA cooperatively with DP proteins through the E2 recognition site, 5'-TTTC[CG]CGC-3' found in the promoter region of a number of genes whose products are involved in cell cycle regulation or in DNA replication. The DRTF1/E2F complex functions in the control of cell-cycle progression from g1 to s phase. E2F-2 binds specifically to RB1 protein, in a cell-cycle dependent manner. PTM: Phosphorylated by CDK2 and cyclin A-CDK2 in the S-phase. similarity: Belongs to the E2F/DP family. subunit: Component of the DRTF1/E2F transcription factor complex. Forms heterodimers with DP family members. The E2F-2 complex binds specifically hypophosphorylated retinoblastoma protein RB1. During the cell cycle, RB1 becomes phosphorylated in mid-to-late G1 phase, detaches from the DRTF1/E2F complex, rendering E2F transcriptionally active. Viral oncoproteins, notably E1A, T-antigen and HPV E7, are capable of sequestering RB protein, thus releasing the active complex. Binds EAPP. tissue specificity: Highest level of expression is found in placenta, low levels are found in lung. Found as well in many immortalized cell lines derived from tumor samples.

• Synonyms:

  Transcription factor E2F2 (E2F-2)

• Gene ID:

  1870

• UniProt:

  Q14209

• Cellular Locus:

  Nucleus.

• Host:

  Rabbit

• Species Reactivity:

  Human, Mouse

• Reactivity:

  Human; Mouse

• Immunogen:

  Synthesized peptide derived from human E2F-2 (Acetyl Lys122)

• Clonality:

  Polyclonal

• Isotype:

  IgG

• Source:

  Rabbit

• Applications:

  WB, ELISA

• Validated Applications:

  WB, ELISA

• Stability:

  -20°C/1 year

• Concentration:

  1 mg/mL

• Dilution:

  WB 1:1000-2000 ELISA 1:5000-20000

• Molecular Weight:

  48kD

• Storage Conditions:

  PBS with 0.02% sodium azide and 50% glycerol pH 7.4. Store at -20°C. Avoid repeated freeze-thaw cycles.

• Observed Molecular Weight:

  48 kD

• Subcellular Location:

  Nucleus.

• Other Product Names:

  Transcription factor E2F2 (E2F-2)

• Gene ID (Human):

  1870

• SwissProt (Human):

  Q14209