Anti-Calponin-1(CALP), CF594 conjugate

Availability: 24/48H Stock Items & 2 to 6 Weeks non Stock Items.
Dry Ice Shipment: No

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Custom Services: We offer custom development, testing, and other tailored services for Anti-Calponin-1(CALP), CF594 conjugate to help researchers meet specific project requirements with precision.
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Anti-Calponin-1(CALP), CF594 conjugate

CAT:

37-BNC940831-100

Size:

100 µL

Anti-Calponin-1(CALP), CF594 conjugate

Description: Calponin is a calmodulin, F-actin and tropomyosin binding protein, which is thought to be involved in the regulation of smooth muscle contraction. Calponin expression is restricted to smooth muscle cells and has been shown to be a marker of the differentiated (contractile) phenotype of developing smooth muscle. Multiple isoelectric variants of calponin have been identified, however only two molecular weight isoforms exist, a 34 kDa form and a 29 kDa form. Expression of the 29 kDa form, I-calponin, is primarily restricted to muscle of the urogenital tract, whereas the higher molecular weight variant has been demonstrated in vascular and visceral smooth muscle. In Western blotting, this MAb reacts with only the 34 kDa form of calponin in extracts of human aortic medial smooth muscle and is unreactive with fibroblast extracts of cultivated human foreskin. Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Synonyms: Calponin-1; CNN1; Calponin 1 basic smooth muscle; Calponin H1 smooth muscle
UNSPSC: 41116161
UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
Gene Name: CNN1
Gene ID: 1264
NCBI Gene ID: 465929
UniProt: P51911
Cellular Locus: Cytoskeleton
Host: Mouse
Species Reactivity: Human, Rat
Immunogen: Crude human uterus extract
Target Antigen: Calponin-1
Clonality: Monoclonal
Isotype: IgG1 κ
Clone: CALP
Conjugation: CF594
Source: Animal
Applications: Flow, intracellular (verified) | IF (verified) | IHC, FFPE (verified) | WB (verified)
Validated Applications: FC, IF, IHC, FFPE, WB
Field of Research: Cytoskeleton
Positive Control: Myoepithelial cells in breast ducts
Concentration: 0.1 mg/mL
Buffer: PBS, 0.1% BSA, 0.05% azide
Molecular Weight: 34 kDa
Additionnal Information: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 1-2 ug/mL|Immunohistology formalin-fixed 0.5-1 ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris with 1 mM EDTA, pH 9.0, for 10-20 min followed by cooling at RT for 20 minutes|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Optimal dilution for a specific application should be determined by user
Shipping Conditions: Room temperature
Storage Conditions: 4°C; Protect from light; Stable at room temperature or 37°C (98°F) for 7 days.
Shelf Life: 2 years

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