Anti-CD27 (Tumor Necrosis Factor Receptor Superfamily 7) (LPFS2/1611)

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Anti-CD27 (Tumor Necrosis Factor Receptor Superfamily 7) (LPFS2/1611)

CAT:

37-BNUB1611-500

Size:

500 µL

Price:

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For price, please contact [email protected].

Anti-CD27 (Tumor Necrosis Factor Receptor Superfamily 7) (LPFS2/1611)

Description: This antibody recognizes a protein of a disulfide-linked 120 kDa dimer, identified as CD27. It is expressed on the majority of peripheral T cells, medullary thymocytes, memory-type B cells, and natural killer cells. It is a transmembrane phosphoglycoprotein that belongs to the tumor necrosis factor receptor (TNFR) superfamily. CD27 binds to its ligand CD70, a member of the TNF family, and induces T-cell co-stimulation and B-cell activation. It also interacts with TRAFs and is involved in activation of NFkB and SAPK/JNK and induces apoptosis.Primary antibodies are available purified, or with a selection of fluorescent CF® Dyes and other labels. CF® Dyes offer exceptional brightness and photostability. Note: Conjugates of blue fluorescent dyes like CF®405S and CF®405M are not recommended for detecting low abundance targets, because blue dyes have lower fluorescence and can give higher non-specific background than other dye colors.
Synonyms: LPFS2; S152; T cell activation antigen S152; T-cell activation antigen CD27; T14; TNFRSF7; TNFSF7; Tp55; Tumor necrosis factor receptor superfamily member 7
UNSPSC: 41116161
UNSPSC Description: Primary and secondary antibodies for multiple methodology immunostaining detection application
Gene Name: CD27
Gene ID: 939
NCBI Gene ID: 355307
UniProt: P26842
Cellular Locus: Plasma membrane
Host: Mouse
Species Reactivity: Human
Immunogen: Recombinant human CD22 protein (exact sequence is proprietary)
Target Antigen: CD27
Clonality: Monoclonal
Isotype: IgG1
Clone: LPFS2/1611
Conjugation: Purified, with BSA
Source: Animal
Applications: Flow (verified) | IF (verified) | IHC, FFPE (verified)
Validated Applications: FC, IF, IHC, FFPE
Field of Research: Immunology
Positive Control: Human peripheral blood lymphocytes. Human tonsil and lymph node.
Concentration: 0.2 mg/mL
Buffer: PBS, 0.05% BSA, 0.05% azide
Molecular Weight: 120 kDa
Additionnal Information: Higher concentration may be required for direct detection using primary antibody conjugates than for indirect detection with secondary antibody|Immunofluorescence: 0.5-1 ug/mL|Immunohistology (formalin): 0.5-1 ug/mL|Staining of formalin-fixed tissues requires boiling tissue sections in 10 mM Tris buffer with 1 mM EDTA pH 9.0 for 10-20 min followed by cooling at RT for 20 min|Flow Cytometry 0.5-1 ug/million cells/0.1 mL|Optimal dilution for a specific application should be determined by user
Shipping Conditions: Room temperature
Storage Conditions: 4°C; Stable at room temperature or 37°C (98°F) for 7 days.
Shelf Life: 2 years

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