His-tag Elution Buffer

• Description:

  His-tag Elution Buffer _x000D_ Catalog number: B2016356_x000D_ Lot number: Batch Dependent_x000D_ Expiration Date: Batch dependent_x000D_ Amount: 50 mL_x000D_ Molecular Weight or Concentration: N/A_x000D_ Supplied as: Solution_x000D_ Applications: a molecular tool for various biochemical applications_x000D_ Storage: RT_x000D_ Keywords: His Elution Buffer_x000D_ Grade: Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered through 0.22 um._x000D_ _x000D_ References:_x000D_ 1: Sensi S, Goebel A. Human Auto-IgG Purification from High Volume Serum Sample by Protein G Affinity Purification Bio Protoc. 2022 Dec 5;12(23):e4562._x000D_ 2: Kish WS, Roach MK, Sachi H, Naik AD, Menegatti S, Carbonell RG. Purification of human erythropoietin by affinity chromatography using cyclic peptide ligands J Chromatogr B Analyt Technol Biomed Life Sci. 2018 May 15;1085:1-12._x000D_ 3: Kenna JG, Major GN, Williams RS. Methods for reducing non-specific antibody binding in enzyme-linked immunosorbent assays J Immunol Methods. 1985 Dec 27;85(2):409-19._x000D_ 4: Mechetner L, Sood R, Nguyen V, Gagnon P, Parseghian MH. The effects of hitchhiker antigens co-eluting with affinity-purified research antibodies J Chromatogr B Analyt Technol Biomed Life Sci. 2011 Sep 1;879(25):2583-94._x000D_ 5: Zhao JC, Zhao ZD, Wang W, Gao XM. Prokaryotic expression, refolding, and purification of fragment 450-650 of the spike protein of SARS-coronavirus Protein Expr Purif. 2005 Feb;39(2):169-74._x000D_ 6: Volpe G, Sozzo U, Piermarini S, Delibato E, Palleschi G, Moscone D. Towards the development of a single-step immunosensor based on an electrochemical screen-printed electrode strip coupled with immunomagnetic beads Anal Bioanal Chem. 2013 Jan;405(2-3):655-63._x000D_ 7: Swartz AR, Xu X, Traylor SJ, Li ZJ, Chen W. One-step affinity capture and precipitation for improved purification of an industrial monoclonal antibody using Z-ELP functionalized nanocages Biotechnol Bioeng. 2018 Feb;115(2):423-432._x000D_ 8: Husby S, Holmskov-Nielsen U, Jensenius JC, Erb K. Increased non-specific binding of heat-treated proteins to plastic surfaces analyzed by ELISA and HPLC-fractionation J Immunoassay. 1985;6(1-2):95-110._x000D_ 9: Kiess W, Kessler U, Funk B, Hoeflich A. Perturbation of C6 glial cells by acetate leads to modulation of [125I]IGF-II binding to the IGF-II/M6P receptor: implications for ligand internalization studies Biochim Biophys Acta. 1994 Sep 8;1223(2):179-84._x000D_ 10: Schreiner U, Theune M, Althof F, Kehm E, Knopf CW. One-step column purification of herpes simplex virus 1 helicase-primase subcomplex using C-terminally his-tagged UL5 subunit Virus Genes. 2009 Aug;39(1):19-29. _x000D_ _x000D_ Products Related to His-tag Elution Buffer can be found at Chemicals

• Short Description:

  Catalog Number: B2016356 (50 mL)_x000D_ His-tag Elution Buffer is a high quality buffer solution used in protein purification techniques, particularly in affinity chromatography, to specifically elute or release proteins that have been bound to a column through a histidine (His)-tag. This product has been used as a molecular tool for various biochemical applications. It has also been used in a wide array of other chemical and immunological applications. Custom bulk amounts of this product are available upon request._x000D_ _x000D_

• Weight:

  2.6

• Length:

  3.3

• Width:

  1.2

• Height :

  1.2