GST-tag Elution Buffer

• Description:

  GST-tag Elution Buffer _x000D_ Catalog number: B2016310_x000D_ Lot number: Batch Dependent_x000D_ Expiration Date: Batch dependent_x000D_ Amount: 50 mL_x000D_ Molecular Weight or Concentration: N/A_x000D_ Supplied as: Powder_x000D_ Applications: a molecular tool for various biochemical applications_x000D_ Storage: ˆ’20°C_x000D_ Keywords: GSH_x000D_ Grade: Biotechnology grade. All products are highly pure. All solutions are made with Type I ultrapure water (resistivity >18 MΩ-cm) and are filtered through 0.22 um._x000D_ _x000D_ References:_x000D_ 1: Schäfer F, Seip N, Maertens B, Block H, Kubicek J. Purification of GST-Tagged Proteins Methods Enzymol. 2015;559:127-39._x000D_ 2: Luo L, King NP, Yeo JC, Jones A, Stow JL. Single-step protease cleavage elution for identification of protein-protein interactions from GST pull-down and mass spectrometry Proteomics. 2014 Jan;14(1):19-23._x000D_ 3: Greenfield EA, DeCaprio J, Brahmandam M. Preparing GST-, His-, or MBP-Fusion Proteins from Bacteria Cold Spring Harb Protoc. 2020 Sep 1;2020(9):100024._x000D_ 4: Harper S, Speicher DW. Purification of proteins fused to glutathione S-transferase Methods Mol Biol. 2011;681:259-80._x000D_ 5: Deceglie S, Lionetti C, Roberti M, Cantatore P, Polosa PL. Expression and purification of large active GST fusion enzymes Methods Mol Biol. 2014;1129:169-80._x000D_ 6: Zwicker N, Adelhelm K, Thiericke R, Grabley S, Hänel F. Strep-tag II for one-step affinity purification of active bHLHzip domain of human c-Myc Biotechniques. 1999 Aug;27(2):368-75._x000D_ 7: Di Tomasso G, Dagenais P, Desjardins A, Rompré-Brodeur A, Delfosse V, Legault P. Affinity purification of RNA using an ARiBo tag Methods Mol Biol. 2012;941:137-55._x000D_ 8: Lichty JJ, Malecki JL, Agnew HD, Michelson-Horowitz DJ, Tan S. Comparison of affinity tags for protein purification Protein Expr Purif. 2005 May;41(1):98-105._x000D_ 9: CzepczyÅ„ska-Krężel H, Czerwinski M, Krężel A, Krop-Watorek A. Isolation of carcinoembryonic antigen N-terminal domains (N-A1) from soluble aggregates Protein Expr Purif. 2011 Jul;78(1):78-85._x000D_ 10: Vinckier NK, Chworos A, Parsons SM. Improved isolation of proteins tagged with glutathione S-transferase Protein Expr Purif. 2011 Feb;75(2):161-4. _x000D_ _x000D_ Products Related to GST-tag Elution Buffer can be found at Chemicals

• Short Description:

  Catalog Number: B2016310 (50 mL)_x000D_ GST-tag Elution Buffer is a high quality buffer solution used in protein purification processes to elute or release a target protein that is fused with a Glutathione S-transferase (GST) tag. This product has been used as a molecular tool for various biochemical applications. It has also been used in a wide array of other chemical and immunological applications. Custom bulk amounts of this product are available upon request._x000D_ _x000D_

• Weight:

  2.6

• Length:

  3.3

• Width:

  1.2

• Height :

  1.2