DAPI

DAPI

• Description: DAPI (4',6-Diamidino-2-Phenylindole, dihydrochloride) is a popular blue DNA dye that is used as a nuclear counterstain in fluorescence microscopy, chromosome staining and flow cytometry. The dye binds to the minor groove of dsDNA with approximately 20-fold fluorescence enhancement, with higher affinity for A-T rich regions. Also see DAPI dilactate, a more water soluble form of the dye.

  • Classic blue nuclear counterstain for fixed cells
  • Can be used at higher concentrations to stain live cells
  • lamba;_Ex/ lamba;_Em = 358/461 nm (with DNA)
  • Off-white solid soluble in H₂O
  • Store at 4°C and protect from light, especially in solution
  • C₁₆H₁₇Cl₂N₅
  • MW = 350.25
  • [28718-90-3]

• CAS Number: 28718-90-3
• UNSPSC: 41116113
• UNSPSC Description: Cytology reagents or solutions or stains
• Source: Synthetic
• Storage Conditions: 4°C; Store with desiccant; Protect from light
• Shelf Life: 12 months