DAPI, 10mg/mL in H2O

DAPI, 10mg/mL in H2O

• Description: DAPI (4',6-Diamidino-2-Phenylindole) is a popular blue DNA dye that is used as a nuclear counterstain in fluorescence microscopy, chromosome staining and flow cytometry. The dye binds to the minor groove of dsDNA with approximately 20-fold fluorescence enhancement, with higher affinity for A-T rich regions. Because of the potential toxicity of DAPI, we offer DAPI dilactate dissolved in H₂O as a safer alternative to weighing out the powdery form. We also offer DAPI dilactate solid, which is more water soluble than the dihydrochloride form of the dye.

  • Classic blue nuclear counterstain for fixed cells
  • Can be used at higher concentrations to stain live cells
  • lamba;_Ex/ lamba;_Em (with DNA) = 358/461 nm
  • Yellow solution
  • Store at 4°C and protect from light
  • C₂₂H₂₇N₅O₆
  • MW: 457.49

• CAS Number: 28718-91-4
• UNSPSC: 41116113
• UNSPSC Description: Cytology reagents or solutions or stains
• Source: Synthetic
• Storage Conditions: 4°C; Protect from light
• Shelf Life: 12 months