Login

NucView® 530 Caspase-3 Substrate, 1 mM in 1X PBS

CAT:
37-10408
Size:
100 µL
Price:
Ask
For price, please contact [email protected]
  • Availability: 24/48H Stock Items & 2 to 6 Weeks non Stock Items.
  • Dry Ice Shipment: No
NucView® 530 Caspase-3 Substrate, 1 mM in 1X PBS - image 1
NucView® 530 Caspase-3 Substrate, 1 mM in 1X PBS - image 2
NucView® 530 Caspase-3 Substrate, 1 mM in 1X PBS - image 3
NucView® 530 Caspase-3 Substrate, 1 mM in 1X PBS - image 4
NucView® 530 Caspase-3 Substrate, 1 mM in 1X PBS - image 5
NucView® 530 Caspase-3 Substrate, 1 mM in 1X PBS - image 6
NucView® 530 Caspase-3 Substrate, 1 mM in 1X PBS - image 7
NucView® 530 Caspase-3 Substrate, 1 mM in 1X PBS - image 8
NucView® 530 Caspase-3 Substrate, 1 mM in 1X PBS - image 9
NucView® 530 Caspase-3 Substrate, 1 mM in 1X PBS - image 10
NucView® 530 Caspase-3 Substrate, 1 mM in 1X PBS - image 11
NucView® 530 Caspase-3 Substrate, 1 mM in 1X PBS - image 12
NucView® 530 Caspase-3 Substrate, 1 mM in 1X PBS - image 13
NucView® 530 Caspase-3 Substrate, 1 mM in 1X PBS - image 14
Thumbnail 1
Thumbnail 2
Thumbnail 3
Thumbnail 4
Thumbnail 5
Thumbnail 6
Thumbnail 7
Thumbnail 8
Thumbnail 9
Thumbnail 10
Thumbnail 11
Thumbnail 12
Thumbnail 13
Thumbnail 14

NucView® 530 Caspase-3 Substrate, 1 mM in 1X PBS

  • Description: NucView® 530 Caspase-3 Substrate, 1 mM in PBS, provides a convenient tool for detecting apoptosis in intact cells based on caspase-3/7 activity using either confocal microscopy or flow cytometry. In contrast to other fluorogenic caspase substrates or fluorescent caspase inhibitor based (FLICA) assays, NucView® caspase-3 substrates can be used to detect caspase-3/7 activity within individual intact cells without inhibiting apoptosis progression. NucView® substrates consist of a fluorogenic DNA dye coupled to the caspase-3/7 DEVD recognition sequence. The substrate, which is initially non-fluorescent, penetrates the plasma membrane and enters the cytoplasm. In apoptotic cells, caspase-3/7 cleaves the substrate, releasing the high-affinity DNA dye, which migrates to the cell nucleus and stains DNA with fluorescence. Thus, NucView® Caspase-3 Substrates are bifunctional, allowing detection of caspase-3/7 activity and visualization of morphological changes in the nucleus during apoptosis. The staining is also formaldehyde-fixable.

    NucView® 530 Caspase-3 Substrate stains apoptotic cell nuclei with orange fluorescence (Ex/Em 528/563 nm), for detection in the Cy®3 channel by fluorescence microscopy (Figure 2) or the PE channel by flow cytometry. NucView® 530 can be used for multi-color imaging with blue, green, or far-red fluorescent probes. Note that when excited by the 488 nm laser line, NucView® 530 also fluoresces in the FITC channel, and therefore cannot be analyzed together with green probes by flow cytometry.

    NucView® 530 Caspase-3 Substrate also is available as a 1 mM solution in DMSO. The substrate in PBS is formulated for use in cells that are sensitive to DMSO toxicity. In non-DMSO sensitive cell types, adding DMSO during the substrate incubation may enhance NucView® 530 staining. Biotium also offers blue fluorogenic NucView® 405 Caspase-3 Substrate, and green fluorogenic NucView® 488 Caspase-3 Substrate and kits.

    Cy dye is a registered trademark of GE Healthcare.
  • UNSPSC: 41116113
  • UNSPSC Description: Cytology reagents or solutions or stains
  • Source: Synthetic
  • Storage Conditions: -35°C; Protect from light
  • Shelf Life: 6 months

Datasheet

MSDS