Recombinant E.coli Lactose Operon Repressor/LacI Protein (His Tag)

Recombinant E.coli Lactose Operon Repressor/LacI Protein (His Tag)

• Background: Lactose operon repressor (LacI) contains one HTH lacI-type DNA-binding domain, functions as a homotetramer. Lactose operon repressor as a repressor of the lactose operon, which also as an inducer, binds allolactose. If remove residues 1-59, resulting the loss of DNA-binding activity but retains tetrameric structure and inducer-binding activity. If delete residues 340-360, resulting the loss of tetramer formation, but retains dimer formation, inducer-binding activity, and DNA-binding activity.
• Abbreviation: LacI
• Synonyms: LacI, Lactose operon repressor
• CAS Number: 9000-83-3
• UniProt: P03023
• Accession Number: P03023
• Host: E. coli
• Origin Species: E.coli
• Tag: C-His
• Sequence: Met1-Gln360
• Purity: > 95% as determined by reducing SDS-PAGE.
• Bioactivity: Not validated for activity
• Reconstitution: It is recommended that sterile water be added to the vial to prepare a stock solution of 0.5 mg/mL. Concentration is measured by UV-Vis.
• Molecular Weight: 40 kDa
• Shipping Conditions: This product is provided as liquid. It is shipped at frozen temperature with blue ice/gel packs. Upon receipt, store it immediately at < - 20°C.
• Storage Conditions: Generally, lyophilized proteins are stable for up to 12 months when stored at -20 to -80°C. Reconstituted protein solution can be stored at 4-8°C for 2-7 days. Aliquots of reconstituted samples are stable at < -20°C for 3 months.