Sheep anti EBNA 3B

• Background:

  EBNA-3B is one of only 10 viral proteins expressed in EBV-transformed B lymphoblasts cultured in vitro, through the expression of EBNA 3B is not required for EBV-mediated transformation in vitro. This 165kDa nuclear protein functions as a transcription factor.

• Synonyms:

  EBNA3B, Epstein-Barr Virus nuclear Antigen 4

• UniProt:

  P03203 (EBV/HHV4)

• Host:

  Sheep

• Species Reactivity:

  Epstein-Barr Virus

• Isotype:

  IgG

• Conjugation:

  Unconjugated

• Type:

  Polyclonal Antibody

• Source:

  Sheep were immunized with recombinant EBNA-3B protein

• Applications:

  ELISA, ICC, IHC (paraffin), Dot blot, WB

• Field of Research:

  Infectious Agents and Disease, Microbiology

• Purification Method:

  Ammonium sulfate precipitation

• Assay Principle:

  Western blotting at 1-5 µg/mL

• Stability:

  See expiration date on vial

• Concentration:

  See vial for Concentration

• Form:

  Provided as solution in phosphate buffered saline with 0,08% sodium azide

• Precautions:

  This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals.

• References & Citations:

  1. Anderton, E., et al, 'Two Epstein-Barr virus (EBV) oncoproteins cooperate to repress expression of the proapoptotic tumour-suppressor Bim: clues to the pathogenesis of Burkitt's lymphoma' Oncogene 2007, 27, , 421-433_x000D_ 2. O'Nions, J., et al, 'Epstein–Barr virus can inhibit genotoxin-induced G1 arrest downstream of p53 by preventing the inactivation of CDK2' Oncogene 2003, 22, , 7181-7191_x000D_ 3. Jiménez-Ramírez, C., et al, 'Epstein-Barr Virus EBNA-3C Is Targeted to and Regulates Expression from the Bidirectional LMP-1/2B Promoter' Journal of Virology 2006, 80, , 11200-11208_x000D_ 4. Yuan, J., et al, 'Virus and Cell RNAs Expressed during Epstein-Barr Virus Replication' Journal of Virology 2006, 80, , 2548-2565_x000D_ 5. Hong, G.K., et al, 'Epstein-Barr Virus Lytic Infection Contributes to Lymphoproliferative Disease in a SCID Mouse Model' Journal of Virology 2005, 79, , 13993-14003_x000D_ 6. Rosendorff, A., et al, 'EBNA3C Coactivation with EBNA2 Requires a SUMO Homology Domain' Journal of Virology 2004, 78, , 367-377_x000D_ 7. Maruo, S., et al, 'Epstein-Barr Virus Nuclear Protein EBNA3A Is Critical for Maintaining Lymphoblastoid Cell Line Growth' Journal of Virology 2003, 77, , 10437-10447_x000D_ 8. Gordadze, A.V., et al, 'Notch1IC Partially Replaces EBNA2 Function in B Cells Immortalized by Epstein-Barr Virus' Journal of Virology 2001, 75, , 5899-5912

• Shipping Conditions:

  Ambient Temperature

• Storage Conditions:

  Product should be stored at -20ºC; Aliquot to avoid freeze/thaw cycles

• Functional Analysis:

  WB

• CAS Number:

  9007-83-4