Sheep anti BrdU (bromodeoxyuridine)

• Background:

  Bromodexyuridine (BrdU) is a thymidine analog which is selectively incorporated into the DNA of proliferating cells to provide a marker for the DNA being replicated. The number of proliferating cells can then be detected in cell lysates, tissue sections or suspensions using an antibody specific for the BrdU. Previous methods of detecting DNA included the use of [3H]-thymidine which would be incorporated into the DNA and could then the DNA could be quantified by autoradiography or scintillation counting. These methods are more difficult and require more cleanup due to the radioactive material. An immunohistochemical assay provides a much simpler and cleaner method for detecting DNA in cells. This antibody is also useful for detecting proliferating cells by flow cytometry or immunofluorescence staining.

• Host:

  Sheep

• Isotype:

  IgG

• Conjugation:

  Unconjugated

• Type:

  Polyclonal Antibody

• Source:

  Sheep were immunized with bromodeoxyuridine conjugated to Helix Pomatia Haemocyanin.

• Applications:

  WB, IP, Flow Cytometry, IHC (frozen and paraffin)

• Field of Research:

  Cell Cycle

• Purification Method:

  Ammonium Sulfate Precipitation

• Assay Principle:

  Applications: Immunoprecipitation: 25-100 µg/mL; Immunohistochemistry: 25-100 µg/mL. The antibody has been tested using immunoprecipitation against 5-methyl cytosine (5-MeC) and bromodeoxyuridine (BrdU) or a control (no antigen). At a concentration of 25 µg/mL, this product demonstrates 8-fold higher reactivity with 5-MeC versus BrdU. For best results, use product at 25-100µg/mL. Note: Denature DNA sample first so that bases are accessible to anti BrdU antibody._x000D_ Applicable in flow cytometry for the quantification of Brdu labeled cells, indication the proliferative activity of the tested cell population.

• Stability:

  See expiration date on vial

• Concentration:

  See vial for Concentration

• Form:

  Provided as solution in phosphate buffered saline with 0,08% sodium azide

• Precautions:

  This product is intended FOR RESEARCH USE ONLY, and FOR TESTS IN VITRO, not for use in diagnostic or therapeutic procedures involving Humans or animals.

• References & Citations:

  1. Nakamura, S., et al. Application of bromodeoxyuridine (BrdU) and anti-BrdU monoclonal antibody for the in vivo analysis of proliferative characteristics of Human leukemia cells in bone marrows. Oncology 1991, 48, 285-289 _x000D_ _x000D_ 2. Wilson, G., Cell kinetic studies using a monoclonal antibody to bromodeoxyuridine. Methods Mol. Biol. 1998, 80, 255-266_x000D_ 3. Gray, J. (Ed), Special Issue: Monoclonal antibodies against bromodeoxyuridine. Cytometry 1985, Vol. 6(6)

• Shipping Conditions:

  Ambient Temperature

• Storage Conditions:

  Product should be stored at -20ºC; Aliquot to avoid freeze/thaw cycles

• Functional Analysis:

  IHC on BrdU-labeled cells

• CAS Number:

  9007-83-4