PD-L1 antibody

CAT: 0013-GTR18398522-01Size: 0.1 mLDry Ice: NoHazardous: No

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CAT#:0013-GTR18398522-01Size:0.1 mL

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24/48H Stock Items & 2 to 6 Weeks non Stock Items.

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Description

PD-L1 antibody validated for immunohistochemistry on 76 different Normal Tissues

Product Name Alternative

B7 homolog 1; B7-H1; CD274; PD-L1; PDCD1 ligand 1; PDCD1L1; PDCD1LG1; Programmed cell death 1 ligand 1

UniProt

Q9NZQ7

Reactivity

Human

Immunogen

KLH-conjugated linear peptide corresponding to human PD-L1

Clonality

Monoclonal

Clone

MSVA-711R

Conjugation

Unconjugated

Field of Research

Immunology & Inflammation

Dilution

1:100-1:200

Storage Conditions

Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.

Notes

For research use only.

Applications Notes

Positive Control: Alveolar macrophages, tonsil crypt epithelium and the placental cytotrophoblast show strong, predominantly membranous positivity. Follicle centre macrophages should show at least moderate intensity staining. Negative Control: Appendix (negative staining of all epithelial cells) Cellular Localization: Predominantly membranous Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining) . Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.

Tested Applications

IHC

Host or Source

Rabbit

Isotype

IgG

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