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Calbindin 1 antibody

CAT: 0013-GTR18398419-01Size: 0.1 mLDry Ice: NoHazardous: No
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CAT#:0013-GTR18398419-01Size:0.1 mL
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Description
Calbindin 1 antibody validated for immunohistochemistry on 76 different Normal Tissues
Product Name Alternative
Avian-type; CAB27; CALB 1; CALB; CALB1; CALB1_HUMAN; Calbindin 1 28kDa; Calbindin; Calbindin D28; D 28K; D-28K; D28K; OTTHUMP00000166027; OTTHUMP00000225441; RTVL H protein; Vitamin D dependent calcium binding protein; Vitamin D dependent calcium binding protein avian type; Vitamin D-dependent calcium-binding protein
UniProt
P05937
Reactivity
Human
Immunogen
Recombinant fragment (around aa 7-96) of human CALB1 protein (exact sequence is proprietary)
Clonality
Monoclonal
Clone
MSVA-471M
Conjugation
Unconjugated
Dilution
1:50-1:100
Storage Conditions
Maintain refrigerated at 2-8°C for up to 2 weeks. For long term storage store at -20°C in small aliquots to prevent freeze-thaw cycles.
Notes
For research use only.
Applications Notes
Positive Control: Kidney: A subset of the cells of distal tubuli should show an intense cytoplasmic calbindin 1 immunostaining. Negative Control: Colon: Calbindin 1 immunostaining should be completely absent in all cells. Cellular Localization: Cytoplasmic Protocol Recommendations: Manual Protocol: Freshly cut sections should be used (less than 10 days between cutting and staining) . Heat-induced antigen retrieval for 5 minutes in an autoclave at 121°C in pH 7.8 Target Retrieval Solution buffer. Apply the antibody at a dilution of 1:150 at 37°C for 60 minutes. Visualization of bound antibody by the EnVision Kit (Dako, Agilent) according to the manufacturer’s directions.
Tested Applications
IHC
Host or Source
Mouse
Isotype
IgG2c, kappa

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