Calbindin Antibody / CALB1

• Description:

  The family of EF-hand type Ca2+-binding proteins includes Calbindin D28K, Calbindin D9K, S-100 (also designated oncomodulin) . Calbindin D28K, also known as calbindin, CALB1, D-28K or vitamin D-dependent calcium-binding protein, is a 261-amino acid protein with 6 EF-hand domains, 4 of which are active calcium-binding domains. Expressed in brain, ovary, uterus, testis, pancreas, liver, kidney and intestine, Calbindin D28K acts as a calcium-buffering agent and alters the activity of the plasma membrane ATPase. In neuronal cells, Calbindin D28K modulates calcium channel activity, calcium transients and intrinsic neuronal firing activity. Also, Calbindin D28K has been implicated to play a role in apoptosis and microtubule function.

• Specifications:

  ELISA (order BSA-free format for coating), Immunohistochemistry (FFPE) : 1-2 µg/mL

• UniProt:

  P05937

• Host:

  Mouse

• Reactivity:

  Human

• Immunogen:

  A recombinant human partial protein (amino acids 7-96) was used as the immunogen for this Calbindin antibody.

• Clonality:

  Monoclonal

• Isotype:

  IgG2b κ

• Clone:

  CALB1/2782

• Applications:

  ELISA, IHC-P

• Purity:

  Protein G affinity chromatography

• Format:

  Purified

• Buffer:

  1 mg/ml in 1X PBS; BSA free, sodium azide free

• Reconstitution:

  Store the Calbindin antibody at 2-8oC (with azide) or aliquot and store at -20oC or colder (without azide).

• Limitations:

  This Calbindin antibody is available for research use only.

• Storage Conditions:

  Store the Calbindin antibody at 2-8°C (with azide) or aliquot and store at -20°C or colder (without azide) .

• Formulation:

  1 mg/mL in 1X PBS; BSA free, sodium azide free

• Applications Notes:

  Optimal dilution of the Calbindin antibody should be determined by the researcher.

• CAS Number:

  9007-83-4

• Location:

  Cytoplasmic, nuclear, secreted

• Image Legend:

  IHC staining of FFPE human kidney with Calbindin antibody (clone CALB1/2782) . HIER: boil tissue sections in pH 9 10mM Tris with 1mM EDTA for 20 min and allow to cool before testing.