Human CNTF Protein

• Background:

  Ciliary neurotrophic factor (CNTF) is a member of the cytokine family. CNTF is a survival factor for various neuronal cell types. Subsequent studies have demonstrated that CNTF is a survival factor for additional neuronal cell types including: dorsal root ganglion sensory neurons, sympathetic ganglion neurons, embryonic motor neurons, major pelvic ganglion neurons and hippocampal neurons.

• Description:

  Human CNTF protein, expressed in E. coli

• Product Name Alternative:

  Ciliary Neurotrophic Factor; Ciliary Neuronotrophic Factor; HCNTF

• Gene ID:

  1270

• Accession Number:

  P26441

• Tag:

  CNTF

• Type:

  Cytokines and proteins

• Applications:

  No tag

• Purity:

  > 98 % as determined by SDS-PAGE

• Activity:

  Measured in a cell proliferation assay using TF-1. The ED50 for this effect is typically 0.35 ng/mL. The specific activity of recombinant Human CNTF is approximately >2.86 x 106 IU/mg.

• Molecular Weight:

  22 kDa

• Precautions:

  The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.

• Features & Benefits:

  Endotoxin: < 0.1 EU per µg of the protein as determined by the LAL method.

• Shipping Conditions:

  Gel pack with blue ice.

• Storage Conditions:

  Lyophilized Human CNTF proteinshould be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) . Please prevent freeze-thaw cycles.

• Protein Length:

  The recombinant Human CNTF consists of 199 amino acids and has a predicted molecular mass of 22 kDa.

• Formulation:

  Lyophilized from sterile PBS, pH 7.5.

• Applications Notes:

  Human

• Other Product Names:

  Ciliary Neurotrophic Factor; Ciliary Neuronotrophic Factor; HCNTF

• Recommended Usage:

  Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Human CNTF protein using the buffer we provided not less than 100 µg/mL, which can then be further diluted to other aqueous solutions.

• Preparation:

  E. coli

• Accession Number URL:

  https://www.uniprot.org/uniprot/P26441

• AA Sequence:

  Amino acid sequence derived from Human CNTF (P26441) (Ala2-Met200) was expressed.