Products for Research Use Only

Human CNTF Protein

CAT: 0749-PRP1002-01Size: 5 µgDry Ice: NoHazardous: No
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CAT#:0749-PRP1002-01Size:5 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
Ciliary neurotrophic factor (CNTF) is a member of the cytokine family. CNTF is a survival factor for various neuronal cell types. Subsequent studies have demonstrated that CNTF is a survival factor for additional neuronal cell types including: dorsal root ganglion sensory neurons, sympathetic ganglion neurons, embryonic motor neurons, major pelvic ganglion neurons and hippocampal neurons.
Description
Human CNTF protein, expressed in E. coli
Product Name Alternative
Ciliary Neurotrophic Factor; Ciliary Neuronotrophic Factor; HCNTF
Gene ID
1270
Accession Number
P26441
Tag
CNTF
Type
Cytokines and proteins
Applications
No tag
Purity
> 98 % as determined by SDS-PAGE
Activity
Measured in a cell proliferation assay using TF-1. The ED50 for this effect is typically 0.35 ng/mL. The specific activity of recombinant Human CNTF is approximately >2.86 x 106 IU/mg.
Molecular Weight
22 kDa
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Features & Benefits
Endotoxin: < 0.1 EU per µg of the protein as determined by the LAL method.
Shipping Conditions
Gel pack with blue ice.
Storage Conditions
Lyophilized Human CNTF proteinshould be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) . Please prevent freeze-thaw cycles.
Protein Length
The recombinant Human CNTF consists of 199 amino acids and has a predicted molecular mass of 22 kDa.
Formulation
Lyophilized from sterile PBS, pH 7.5.
Applications Notes
Human
Other Product Names
Ciliary Neurotrophic Factor; Ciliary Neuronotrophic Factor; HCNTF
Recommended Usage
Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Human CNTF protein using the buffer we provided not less than 100 µg/mL, which can then be further diluted to other aqueous solutions.
Preparation
E. coli
Accession Number URL
https://www.uniprot.org/uniprot/P26441
AA Sequence
Amino acid sequence derived from Human CNTF (P26441) (Ala2-Met200) was expressed.