Human IFN-γ Protein

IFN-γ, also known as IFNG, is a secreted protein which belongs to the type I I interferon family. IFN-γ is produced predominantly by natural killer and natural killer T cells as part of the innate immune response, and by CD4 and CD8 cytotoxic T lymphocyte effector T cells once antigen-specific immunity develops. IFN-γ has antiviral, immunoregulatory, and anti-tumor properties. IFNG, in addition to having antiviral activity, has important immunoregulatory functions, it is a potent activator of macrophages, and has antiproliferative effects on transformed cells and it can potentiate the antiviral and antitumor effects of the type I interferons. The IFNG monomer consists of a core of six α-helices and an extended unfolded sequence in the C-terminal region. IFN-γ is critical for innate and adaptive immunity against viral and intracellular bacterial infections and for tumor control. Aberrant IFN-γ expression is associated with a number of autoinflammatory and autoimmune diseases. The importance of IFN-γ in the immune system stems in part from its ability to inhibit viral replication directly, and most importantly from its immunostimulatory and immunomodulatory effects. IFNG also promotes NK cell activity.

Human IFN-γ protein, His Tag, expressed in E. coli

IFG; IFI; IFN gamma; Interferon Gamma; Interferon gamma; IFNG

3458

P01579

IFN-γ

Cytokines and proteins

No tag

> 98 % as determined by SDS-PAGE

Measured in a cytotoxicity assay using L929 mouse fibrosarcoma cells in the presence of the metabolic inhibitor actinomycin D. The ED50 for this effect is typically 168.4 pg/mL. The specific activity of recombinant Human IFN-γ protein is approximately >5.9 x 106 IU/mg

16.91 kDa

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Gel pack with blue ice.

Lyophilized Human IFN-γ protein, His Tagshould be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) . Please prevent freeze-thaw cycles.

The recombinant Human IFN-γ protein consists of 144 amino acids and predicts a molecular mass of 16.91 KDa

Lyophilized from sterile 150mM NaCl, 20mM Tris , pH 7.0.

Human

IFG; IFI; IFN gamma; Interferon Gamma; Interferon gamma; IFNG

Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized Human IFN-gamma protein in sterile ddH2O not less than 100µg/ml, which can then be further diluted to other aqueous solutions.

E. coli

http://www.uniprot.org/uniprot/P01579