Products for Research Use Only

Human IFN-α2a Protein

CAT: 0749-PRP1037-01Size: 20 µgDry Ice: NoHazardous: No
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CAT#:0749-PRP1037-01Size:20 µg
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24/48H Stock Items & 2 to 6 Weeks non Stock Items.
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Background
At least 23 different variants of IFN-α are known. The individual proteins have molecular masses between 19-26 kDa and consist of proteins with lengths of 156-166 and 172 amino acids. All IFN-α subtypes possess a common conserved sequence region between amino acid positions 115-151 while the amino-terminal ends are variable. Many IFN-α subtypes only differ in their sequences by one or two positions.Naturally occurring variants also include proteins truncated by 10 amino acids at the carboxy-terminal end. IFNA2 (Interferon Alpha 2) is a Protein Coding gene. This gene is a member of the alpha interferon gene cluster on chromosome 9. The encoded protein is a cytokine produced in response to viral infection. Type I Interferons (IFNs) are well-known cytokines that exert antiviral activity, antitumor activity, and immunomodulatory effects
Description
Human IFN-α2a Protein, expressed in E. coli
Product Name Alternative
IFN-α2a
Gene ID
3440
Accession Number
P01563
Tag
IFN-α2a
Type
Cytokines and proteins
Applications
No tag
Purity
> 99 % as determined by SDS-PAGE
Activity
Measured by a cytotoxicity assay using TF-1 Cells. The ED50 for this effect is 0.8896 ng/mL.
Molecular Weight
19.24kD
Precautions
The product listed herein is for research use only and is not intended for use in human or clinical diagnosis. Suggested applications of our products are not recommendations to use our products in violation of any patent or as a license. We cannot be responsible for patent infringements or other violations that may occur with the use of this product.
Features & Benefits
Endotoxin: < 1 EU per µg of the protein as determined by the LAL method
Shipping Conditions
Gel pack with blue ice.
Storage Conditions
Lyophilized recombinant Human IFN-α2a Protein product should be stored desiccated below -20°C. Upon reconstitution, the protein should be stored at 4°C between 2-7 days and for future use below -20°C. For long term storage it is recommended to add a carrier protein (0.1% HSA or BSA) . Please prevent freeze-thaw cycles.
Protein Length
The recombinant Human IFN-α2a consists of 165 amino acids with 8xHis tag at C terminus and predicts a molecular mass of 19.24 kDa.
Formulation
Lyophilized from 20mM Tris 150mM NaCl, pH 8.0.
Applications Notes
Human
Other Product Names
IFN-α2a
Recommended Usage
Always centrifuge tubes before opening. It is recommended to reconstitute the lyophilized recombinant Human IFN-α2a Protein using the buffer we provided not less than 100µg/ml, which can then be further diluted to other aqueous solutions.
Preparation
E. coli
AA Sequence
Amino acid sequence derived from Human IFN-α2a (P01563) (Cys24-Glu188) was expressed.