MYLK Rabbit Polyclonal Antibody

Myosin light chain kinase, smooth muscle also known as kinase-related protein (KRP) or telokin is an enzyme that in humans is encoded by the MYLK gene. This gene, a muscle member of the immunoglobulin superfamily, encodes a myosin light-chain kinase, which is a calcium-/calmodulin-dependent enzyme. This kinase phosphorylates myosin regulatory light chains to facilitate myosin interaction with actin filaments to produce contractile activity. This gene encodes both smooth muscle and nonmuscle isoforms. In addition, using a separate promoter in an intron in the 3' region, it encodes telokin, a small protein identical in sequence to the C-terminus of myosin light chain kinase, that is independently expressed in smooth muscle and functions to stabilize unphosphorylated myosin filaments. A pseudogene is located on the p arm of chromosome 3. Four transcript variants that produce four isoforms of the calcium/calmodulin dependent enzyme have been identified as well as two transcripts that produce two isoforms of telokin. Additional variants have been identified but lack full length transcripts.

9007-83-4

caMLCK antibody cardiac MLCK antibody Cardiac MyBP C associated Ca/CaM kinase antibody Cardiac-MyBP-C-associated Ca/CaM kinase antibody MLC kinase antibody MLCK antibody MLCK2 antibody Mylk3 antibody MYLK3_HUMAN antibody Myosin light chain kinase 3 antibody Putative myosin light chain kinase 3 antibody

MLCK, smMLCK, KRP

Q15746 Human, Q6PDN3 Mouse

Cytoplasm, Cell projection, Cleavage furrow

Recombinant protein with Human MYLK aa 230-470 / 1,914.

Non-conjugated

Rabbit polyclonal Antibody

IF-Cell, IHC-P, FC, WB

MCF-7, SW480, mouse colon tissue, mouse smooth muscle tissue, human kidney tissue lysates.

1 mg/mL

IF-Cell: 1:50-1:200 ;IHC-P: 1:50-1:200 ;FC: 1:10-1:100 ;WB: 1:1,000

Immunogen affinity purified.

Liquid

1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.

Predicted band size: 211 kDa

Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.