GAPDH Recombinant Rabbit Monoclonal Antibody [PD00-07]

GAPDH Recombinant Rabbit Monoclonal Antibody [PD00-07]

• Description: Has both glyceraldehyde-3-phosphate dehydrogenase and nitrosylase activities, thereby playing a role in glycolysis and nuclear functions, respectively. Participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. Nuclear functions are probably due to the nitrosylase activity that mediates cysteine S-nitrosylation of nuclear target proteins such as SIRT1, HDAC2 and PRKDC. Modulates the organization and assembly of the cytoskeleton. Facilitates the CHP1-dependent microtubule and membrane associations through its ability to stimulate the binding of CHP1 to microtubules. Glyceraldehyde-3-phosphate dehydrogenase is a key enzyme in glycolysis that catalyzes the first step of the pathway by converting D-glyceraldehyde 3-phosphate (G3P) into 3-phospho-D-glyceroyl phosphate. Component of the GAIT (gamma interferon-activated inhibitor of translation) complex which mediates interferon-gamma-induced transcript-selective translation inhibition in inflammation processes. Upon interferon-gamma treatment assembles into the GAIT complex which binds to stem loop-containing GAIT elements in the 3'-UTR of diverse inflammatory mRNAs (such as ceruplasmin) and suppresses their translation.
• Alternative Name: 38 kDa BFA-dependent ADP-ribosylation substrate antibody aging associated gene 9 protein antibody Aging-associated gene 9 protein antibody BARS-38 antibody cb609 antibody EC 1.2.1.12 antibody Epididymis secretory sperm binding protein Li 162eP antibody G3P_HUMAN antibody G3PD antibody G3PDH antibody GAPD antibody GAPDH antibody Glyceraldehyde 3 phosphate dehydrogenase antibody Glyceraldehyde-3-phosphate dehydrogenase antibody HEL-S-162eP antibody KNC-NDS6 antibody MGC102544 antibody MGC102546 antibody MGC103190 antibody MGC103191 antibody MGC105239 antibody MGC127711 antibody MGC88685 antibody OCAS, p38 component antibody OCT1 coactivator in S phase, 38-KD component antibody peptidyl cysteine S nitrosylase GAPDH antibody Peptidyl-cysteine S-nitrosylase GAPDH antibody wu:fb33a10 antibody
• Abbreviation: GAPDH
• Swiss Prot: P04406 Human, P16858 Mouse, P04797 Rat
• Cellular Locus: Cytoskeleton, nucleus, cytosol, perinuclear region.
• Host: Rabbit
• Species Reactivity: Human, Mouse, Rat, Escherichia coli, Zebrafish, Drosophila melanogaster
• Immunogen: Recombinant protein within mouse GAPDH aa 94-333 / 333.
• Isotype: IgG
• Conjugation: Non-conjugated
• Type: Recombinant Rabbit monoclonal Antibody
• Applications: WB, IHC-P, IF-Cell, FC
• Positive Control: HEK-293 cell lysate, HeLa cell lysate, A431 cell lysate, K-562 cell lysate, RAW264.7 cell lysate, PC-12 cell lysate, mouse heart tissue lysate, rat heart tissue lysate, human liver tissue lysate, mouse liver tissue lysate, rat liver tissue lysate, human kidney tissue lysate, mouse kidney tissue lysate, rat kidney tissue lysate, E.coli cell lysate, HepG2 cell lysates, NIH/3T3 cell lysates, PC-12 cell lysates, zebrafish tissue lysates, human pancreas tissue, human kidney tissue, mouse kidney tissue, rat kidney tissue, HeLa, NIH/3T3.
• Concentration: 1 mg/mL
• Dilution: WB: 1:2,500-1:20,000 ;IHC-P: 1:1,000-1:5,000 ;IF-Cell: 1:1,000 ;FC: 1:1,000
• Purity: Protein A affinity purified.
• Form: Liquid
• Buffer: PBS (pH7.4), 0.1% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
• Molecular Weight: Predicted band size: 36 kDa
• Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
• Recombinant Antibody: Yes