GAPDH Rabbit Polyclonal Antibody

CAT: 0992-R1210-1Size: 100 µLDry Ice: NoHazardous: No
CAT#:0992-R1210-1Size:100 µL
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Description
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH) catalyzes the phosphorylation of glyceraldehyde-3-phosphate during glycolysis. It participates in nuclear events including transcription, RNA transport, DNA replication and apoptosis. GAPDH is thought to be a constitutively expressed housekeeping protein. For this reason, GAPDH mRNA and protein levels are often measured as controls in experiments quantifying specific changes in expression of other targets.
CAS Number
9007-83-4
Product Name Alternative
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Abbreviation
GAPDH
Swiss Prot
P04406 Human, P16858 Mouse, P46406 Rabbit
Cellular Locus
Cytoplasm, Nucleus.
Host
Rabbit
Species Reactivity
Human, Mouse, Rat, Oryza sativa
Immunogen
This antibody is produced by immunizing rabbits with full length recombinant protein of GAPDH.
Isotype
IgG
Conjugation
Non-conjugated
Type
Rabbit polyclonal Antibody
Applications
WB, IF-Cell, IHC-P, FC
Positive Control
HeLa cell lysate, A431 cell lysate, HEK-293 cell lysate, Jurkat cell lysate, NIH/3T3 cell lysate, RAW264.7 cell lysate, PC-12 cell lysate, C6 cell lysate, mouse brain tissue lysate, rat brain tissue lysate, A549, LOVO, MCF-7, rat kidney tissue, human colon cancer tissue, human spleen tissue, mouse testis tissue, Hela.
Concentration
1 mg/mL
Dilution
WB: 1:5,000-1:20,000 ;IF-Cell: 1:100-1:200 ;IHC-P: 1:50-1:200 ;FC: 1:50-1:100
Purity
Immunogen affinity purified.
Form
Liquid
Buffer
1*PBS (pH7.4), 0.2% BSA, 40% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight
Predicted band size: 36 kDa
Storage Conditions
Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
Recombinant Antibody
No

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