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GAPDH Rabbit Polyclonal Antibody

CAT: 0992-ER1706-83Size: 100 µLDry Ice: NoHazardous: No
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CAT#:0992-ER1706-83Size:100 µL
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Description
Glyceraldehyde-3-phosphate dehydrogenase (GAPDH), also called uracil DNA glycosylase, catalyzes the reversible oxidative phosphorylation of glyceraldehyde-3-phosphate in the presence of inorganic phosphate and nicotinamide adenine dinucleotide (NAD), an important energy-yielding step in carbohydrate metabolism. While GAPDH has long been recognized as playing an integral role in glycolysis, additional functions of GAPDH include acting as a uricil DNA glycosylase, activating transcription, binding RNA and involvement in nuclear RNA export, DNA replication and DNA repair. Expression of GAPDH is upregulated in liver, lung and prostate cancers. GAPDH translocates to the nucleus during apoptosis. GAPDH complexes with neuronal proteins implicated in human neuro-degenerative disorders including the β-Amyloid precursor, Huntingtin and other triplet repeat neuronal disorder proteins.
CAS Number
9007-83-4
Product Name Alternative
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Abbreviation
GAPDH, GAPD, CDABP0047, OK/SW-cl.12
Swiss Prot
P04406 Human, P16858 Mouse, P04797 Rat
Cellular Locus
Cytoskeleton. Cytosol. Nucleus.
Host
Rabbit
Species Reactivity
Human, Mouse, Rat
Immunogen
Synthetic peptide within human GAPDH aa 171-226.
Isotype
IgG
Conjugation
Non-conjugated
Type
Rabbit polyclonal Antibody
Applications
WB, IF-Cell, IHC-P, FC
Positive Control
PC-12, L929, NIH/3T3, F9, mouse liver tissue lysate, LOVO , human kidney tissue, mouse kidney tissue, rat kidney tissue, MCF-7.
Concentration
1 mg/mL
Dilution
WB: 1:2,000-5,000 ;IF-Cell: 1:50-1:200 ;IHC-P: 1:200 ;FC: 1:50-1;100
Purity
Immunogen affinity purified.
Form
Liquid
Buffer
1*PBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
Molecular Weight
Predicted band size: 36 kDa
Storage Conditions
Store at +4°C after thawing. Aliquot store at -20°C or -80°C. Avoid repeated freeze / thaw cycles.
Recombinant Antibody
No

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