AP2A2 Rabbit Polyclonal Antibody

AP2A2 Rabbit Polyclonal Antibody

• Description: Component of the adaptor protein complex 2 (AP-2). Adaptor protein complexes function in protein transport via transport vesicles in different membrane traffic pathways. Adaptor protein complexes are vesicle coat components and appear to be involved in cargo selection and vesicle formation. AP-2 is involved in clathrin-dependent endocytosis in which cargo proteins are incorporated into vesicles surrounded by clathrin (clathrin-coated vesicles, CCVs) which are destined for fusion with the early endosome. The clathrin lattice serves as a mechanical scaffold but is itself unable to bind directly to membrane components. Clathrin-associated adaptor protein (AP) complexes which can bind directly to both the clathrin lattice and to the lipid and protein components of membranes are considered to be the major clathrin adaptors contributing the CCV formation. AP-2 also serves as a cargo receptor to selectively sort the membrane proteins involved in receptor-mediated endocytosis. AP-2 seems to play a role in the recycling of synaptic vesicle membranes from the presynaptic surface. AP-2 recognizes Y-X-X-[FILMV] (Y-X-X-Phi) and [ED]-X-X-X-L-[LI] endocytosis signal motifs within the cytosolic tails of transmembrane cargo molecules. AP-2 may also play a role in maintaining normal post-endocytic trafficking through the ARF6-regulated, non-clathrin pathway. During long-term potentiation in hippocampal neurons, AP-2 is responsible for the endocytosis of ADAM10.
• Product Name Alternative: 100 kDa coated vesicle protein C antibody Adaptor protein complex AP-2 subunit alpha-2 antibody Adaptor-related protein complex 2 subunit alpha-2 antibody Alpha-adaptin C antibody Alpha2-adaptin antibody AP-2 complex subunit alpha-2 antibody Ap2a2 antibody AP2A2_HUMAN antibody Clathrin assembly protein complex 2 alpha-C large chain antibody HIP-9 antibody Huntingtin yeast partner J antibody Huntingtin-interacting protein 9 antibody Huntingtin-interacting protein J antibody Plasma membrane adaptor HA2/AP2 adaptin alpha C subunit antibody
• Abbreviation: AP2A2
• CAS Number: 9007-83-4
• Swiss Prot: O94973 Human, P17427 Mouse, P18484 Rat
• Cellular Locus: Cell membrane, coated pit.
• Host: Rabbit
• Species Reactivity: Human, Mouse, Rat
• Immunogen: Recombinant protein within human AP2A2 aa 740-939 / 939.
• Isotype: IgG
• Conjugation: Non-conjugated
• Type: Rabbit polyclonal Antibody
• Applications: WB, IHC-P
• Positive Control: Hela cell lysate, mouse cerebellum tissue lysate, rat brain tissue lysate, human kidney tissue, mouse cerebellum tissue.
• Concentration: 1 mg/mL
• Dilution: WB: 1:500-1:5,000 ;IHC-P: 1:200-1:1,000
• Purity: Immunogen affinity purified.
• Form: Liquid
• Buffer: 1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.
• Molecular Weight: 104 kDa
• Storage Conditions: Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.
• Recombinant Antibody: No