PIP5K1A Rabbit Polyclonal Antibody

Catalyzes the phosphorylation of phosphatidylinositol 4-phosphate (PtdIns4P) to form phosphatidylinositol 4,5-bisphosphate (PtdIns (4,5)P2). PtdIns (4,5)P2 is involved in a variety of cellular processes and is the substrate to form phosphatidylinositol 3,4,5-trisphosphate (PtdIns (3,4,5)P3), another second messenger. The majority of PtdIns (4,5)P2 is thought to occur via type I phosphatidylinositol 4-phosphate 5-kinases given the abundance of PtdIns4P. Participates in a variety of cellular processes such as actin cytoskeleton organization, cell adhesion, migration and phagocytosis. Required for membrane ruffling formation, actin organization and focal adhesion formation during directional cell migration by controlling integrin-induced translocation of RAC1 to the plasma membrane. Together with PIP5K1C is required for phagocytosis, but they regulate different types of actin remodeling at sequential steps. Promotes particle ingestion by activating WAS that induces Arp2/3 dependent actin polymerization at the nascent phagocytic cup. Together with PIP5K1B is required after stimulation of G-protein coupled receptors for stable platelet adhesion. Plays a role during calcium-induced keratinocyte differentiation. Recruited to the plasma membrane by the E-cadherin/beta-catenin complex where it provides the substrate PtdIns (4,5)P2 for the production of PtdIns (3,4,5)P3, diacylglycerol and inositol 1,4,5-trisphosphate that mobilize internal calcium and drive keratinocyte differentiation. Together with PIP5K1C have a role during embryogenesis. Functions also in the nucleus where acts as an activator of TUT1 adenylyltransferase activity in nuclear speckles, thereby regulating mRNA polyadenylation of a select set of mRNAs. Positively regulates insulin-induced translocation of SLC2A4 to the cell membrane in adipocytes (By similarity).

9007-83-4

68 kDa type I phosphatidylinositol-4-phosphate 5-kinase alpha antibody Phosphatidylinositol 4 phosphate 5 kinase type I alpha antibody Phosphatidylinositol-4-phosphate 5-kinase type I alpha antibody Phosphatidylinositol-4-phosphate 5-kinase type-1 alpha antibody PI4P5K Ia antibody PI51A_HUMAN antibody PIP5K A antibody PIP5K1-alpha antibody Pip5k1a antibody PIP5KIalpha antibody PtdIns (4)P-5-kinase 1 alpha antibody

PIP5K1A

Q99755 Human, P70182 Mouse, D3ZSI8 Rat

Cell membrane, Nucleus, Nucleus speckle, Cytoplasm, ruffle, lamellipodium.

Recombinant protein within human PIP5K1A aa 400-562.

Non-conjugated

Rabbit polyclonal Antibody

WB, IHC-P, FC

293T cell lysate, SiHa cell lysate, SK-Br-3 cell lysate, rat skeletal muscle tissue, human tonsil tissue, human liver tissue, mouse testis tissue, SiHa.

1 mg/mL

WB: 1:500-1:1,000 ;IHC-P: 1:100-1:500 ;FC: 1:500-1:1,000

Immunogen affinity purified.

Liquid

1*TBS (pH7.4), 0.2% BSA, 50% Glycerol. Preservative: 0.05% Sodium Azide.

63 kDa

Store at +4°C after thawing. Aliquot store at -20°C. Avoid repeated freeze/thaw cycles.